Intermediate

Part:BBa_K133004:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)

CF-UB33-RGD-Histop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1207
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 764
    Illegal BamHI site found at 1275
    Illegal BamHI site found at 1627
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1721


Design Notes

The CF-UB33-RGD-Histop was cloned into pSB1.AK3 without terminator for use in bacterial cells (protein production).

Source

The composite parts of chimeric protein were amplificated by PCR reaction from E. coli and H. pylori genomic DNA respecively. UB33 is a synthetic gene orderd from GeneArt. Since the protein was designed to be produced, we additionally added RGD-Hisstop tag (for the part description and implication follow: [1]

References